Regulatory

Part:BBa_K5102068:Design

Designed by: Natalia Kuźmierkiewicz   Group: iGEM24_Munich   (2024-09-30)


CMV 5' UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part harbors a point mutation designed to disrupt the BsmBI-v2 recognition site. This modification was made to prevent unintended cleavage during Golden Gate cloning, which relied on BsmBI-v2. Without this disruption, the recognition site could interfere with cloning efficiency and cause issues during plasmid construction.


Source

The part has been amplified from an already existing plasmid in the lab.

References